UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13G Under the Securities Exchange Act of 1934 (Amendment No. 1)* Cholestech Corporation -------------------------------------------------------------------------------- (Name of Issuer) Common Stock -------------------------------------------------------------------------------- (Title of Class of Securities) 170393102 ---------------------------------- (CUSIP Number) August 8, 2006 -------------------------------------------------------------------------------- (Date of Event Which Requires Filing of this Statement) Check the appropriate box to designate the rule pursuant to which this Schedule is filed: [ ] Rule 13d-1(b) [X] Rule 13d-1(c) [ ] Rule 13d-1(d) * The remainder of this cover page shall be filled out for a reporting person's initial filing on this form with respect to the subject class of securities, and for any subsequent amendment containing information which would alter the disclosures provided in a prior cover page. The information required in the remainder of this cover page shall not be deemed to be "filed" for the purpose of Section 18 of the Securities Exchange Act of 1934 ("Act") or otherwise subject to the liabilities of that section of the Act but shall be subject to all other provisions of the Act (however, see the Notes). CUSIP No. 170393102 ------------------------------------------------------------------------------- 1 NAME OF REPORTING PERSON/ I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (Entities Only) Discovery Equity Partners, L.P. ------------------------------------------------------------------------------- 2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP (See Instructions) (a) [ ] Not Applicable (b) [ ] ------------------------------------------------------------------------------- 3 SEC USE ONLY ------------------------------------------------------------------------------- 4 CITIZENSHIP OR PLACE OF ORGANIZATION Illinois ------------------------------------------------------------------------------- 5 SOLE VOTING POWER None ----------------------------------------------------------------- NUMBER OF 6 SHARED VOTING POWER SHARES BENEFICIALLY 750,290 OWNED BY ----------------------------------------------------------------- EACH 7 SOLE DISPOSITIVE POWER REPORTING PERSON None WITH ----------------------------------------------------------------- 8 SHARED DISPOSITIVE POWER 750,290 ------------------------------------------------------------------------------- 9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 750,290 ------------------------------------------------------------------------------- 10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES (see Instructions) [ ] Not Applicable ------------------------------------------------------------------------------- 11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 5.0 % ------------------------------------------------------------------------------- 12 TYPE OF REPORTING PERSON (see Instructions) PN ------------------------------------------------------------------------------- CUSIP No. 170393102 ------------------------------------------------------------------------------- 1 NAME OF REPORTING PERSON/ I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (Entities Only) Discovery Group I, LLC ------------------------------------------------------------------------------- 2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP (See Instructions) (a) [ ] Not Applicable (b) [ ] ------------------------------------------------------------------------------- 3 SEC USE ONLY ------------------------------------------------------------------------------- 4 CITIZENSHIP OR PLACE OF ORGANIZATION Delaware ------------------------------------------------------------------------------- 5 SOLE VOTING POWER None ----------------------------------------------------------------- NUMBER OF 6 SHARED VOTING POWER SHARES BENEFICIALLY 877,695 OWNED BY ----------------------------------------------------------------- EACH 7 SOLE DISPOSITIVE POWER REPORTING PERSON None WITH ----------------------------------------------------------------- 8 SHARED DISPOSITIVE POWER 877,695 ------------------------------------------------------------------------------- 9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 877,695 ------------------------------------------------------------------------------- 10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES (see Instructions) [ ] Not Applicable ------------------------------------------------------------------------------- 11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 5.9 % ------------------------------------------------------------------------------- 12 TYPE OF REPORTING PERSON (see Instructions) OO ------------------------------------------------------------------------------- CUSIP No. 170393102 ------------------------------------------------------------------------------- 1 NAME OF REPORTING PERSON/ I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (Entities Only) Daniel J. Donoghue ------------------------------------------------------------------------------- 2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP (See Instructions) (a) [ ] Not Applicable (b) [ ] ------------------------------------------------------------------------------- 3 SEC USE ONLY ------------------------------------------------------------------------------- 4 CITIZENSHIP OR PLACE OF ORGANIZATION U.S.A. ------------------------------------------------------------------------------- 5 SOLE VOTING POWER None ----------------------------------------------------------------- NUMBER OF 6 SHARED VOTING POWER SHARES BENEFICIALLY 877,695 OWNED BY ----------------------------------------------------------------- EACH 7 SOLE DISPOSITIVE POWER REPORTING PERSON None WITH ----------------------------------------------------------------- 8 SHARED DISPOSITIVE POWER 877,695 ------------------------------------------------------------------------------- 9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 877,695 ------------------------------------------------------------------------------- 10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES (see Instructions) [ ] Not Applicable ------------------------------------------------------------------------------- 11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 5.9 % ------------------------------------------------------------------------------- 12 TYPE OF REPORTING PERSON (see Instructions) IN ------------------------------------------------------------------------------- CUSIP No. 170393102 ------------------------------------------------------------------------------- 1 NAME OF REPORTING PERSON/ I.R.S. IDENTIFICATION NOS. OF ABOVE PERSONS (Entities Only) Michael R. Murphy ------------------------------------------------------------------------------- 2 CHECK THE APPROPRIATE BOX IF A MEMBER OF A GROUP (See Instructions) (a) [ ] Not Applicable (b) [ ] ------------------------------------------------------------------------------- 3 SEC USE ONLY ------------------------------------------------------------------------------- 4 CITIZENSHIP OR PLACE OF ORGANIZATION U.S.A. ------------------------------------------------------------------------------- 5 SOLE VOTING POWER None ----------------------------------------------------------------- NUMBER OF 6 SHARED VOTING POWER SHARES BENEFICIALLY 877,695 OWNED BY ----------------------------------------------------------------- EACH 7 SOLE DISPOSITIVE POWER REPORTING PERSON None WITH ----------------------------------------------------------------- 8 SHARED DISPOSITIVE POWER 877,695 ------------------------------------------------------------------------------- 9 AGGREGATE AMOUNT BENEFICIALLY OWNED BY EACH REPORTING PERSON 877,695 ------------------------------------------------------------------------------- 10 CHECK IF THE AGGREGATE AMOUNT IN ROW (9) EXCLUDES CERTAIN SHARES (see Instructions) [ ] Not Applicable ------------------------------------------------------------------------------- 11 PERCENT OF CLASS REPRESENTED BY AMOUNT IN ROW (9) 5.9 % ------------------------------------------------------------------------------- 12 TYPE OF REPORTING PERSON (see Instructions) IN ------------------------------------------------------------------------------- Item 1. (a) Name of Issuer Cholestech Corporation (b) Address of Issuer's Principal Executive Offices 3347 Investment Boulevard, Hayward, California 94545 Item 2. (a) Name of Person Filing Discovery Equity Partners, L.P. ("Discovery Partners") Discovery Group I, LLC, the general partner of Discovery Partners ("Discovery Group") Daniel J. Donoghue, a Managing Member of Discovery Group Michael R. Murphy, a Managing Member of Discovery Group (b) Address of Principal Business Office or, if none, Residence Discovery Partners, Discovery Group, Mr. Donoghue, and Mr. Murphy are all located at: Hyatt Center, 24th Floor, 71 South Wacker Drive, Chicago, Illinois 60606 (c) Citizenship Discovery Partners is an Illinois limited partnership Discovery Group is a Delaware limited liability company Mr. Donoghue and Mr. Murphy are U.S. citizens (d) Title of Class of Securities Common Stock (e) CUSIP Number 170393102 Item 3. If this statement is filed pursuant to Sections 240.13d-1(b), or 240.13d-2(b) or (c), check whether the person filing is a: Not Applicable (a) [ ] Broker or dealer registered under section 15 of the Act (15 U.S.C. 78o). (b) [ ] Bank as defined in section 3(a)(6) of the Act (15 U.S.C. 78c). (c) [ ] Insurance company as defined in section 3(a)(19) of the Act (15 U.S.C. 78c). (d) [ ] Investment company registered under section 8 of the Investment Company Act of 1940 (15 U.S.C. 80a-8). (e) [ ] An investment adviser in accordance with Section 240.13d-1(b)(1)(ii)(E); (f) [ ] An employee benefit plan or endowment fund in accordance with Section 240.13d-1(b)(1)(ii)(F); (g) [ ] A parent holding company or control person in accordance with Section 240.13d-1(b)(1)(ii)(G); (h) [ ] A savings associations as defined in Section 3(b) of the Federal Deposit Insurance Act (12 U.S.C. 1813); (i) [ ] A church plan that is excluded from the definition of an investment company under section 3(c)(14) of the Investment Company Act of 1940 (15 U.S.C. 80a-3); (j) [ ] Group, in accordance with Section 240.13d-1(b)(1)(ii)(J). Item 4. Ownership Provide the following information regarding the aggregate number and percentage of the class of securities of the issuer identified in Item 1. (a) Amount beneficially owned: Discovery Partners - 750,290 Discovery Group - 877,695 Mr. Donoghue - 877,695 Mr. Murphy - 877,695 (b) Percent of class: Discovery Partners - 5.0 % Discovery Group - 5.9 % Mr. Donoghue - 5.9 % Mr. Murphy - 5.9 % The foregoing percentages are based on 14,988,875 shares of Common Stock of the Issuer identified in Item 1 outstanding as of July 31, 2006, as reported in the Issuer's Quarterly Report on Form 10-Q for the fiscal quarter ended June 30, 2006. (c) Number of shares as to which the person has: (i) Sole power to vote or to direct the vote None (ii) Shared power to vote or to direct the vote Discovery Partners - 750,290 Discovery Group - 877,695 Mr. Donoghue - 877,695 Mr. Murphy - 877,695 (iii) Sole power to dispose or to direct the disposition of None (iv) Shared power to dispose or to direct the disposition of Discovery Partners - 750,290 Discovery Group - 877,695 Mr. Donoghue - 877,695 Mr. Murphy - 877,695 Item 5. Ownership of Five Percent or Less of a Class If this statement is being filed to report the fact that as of the date hereof the reporting person has ceased to be the beneficial owner of more than five percent of the class of securities, check the following. Not Applicable Item 6. Ownership of More than Five Percent on Behalf of Another Person The shares reported herein have been acquired on behalf of discretionary clients of Discovery Group, including Discovery Partners. Such discretionary clients are entitled to receive all dividends from, and proceeds from the sale of, those shares. Except for Discovery Partners, none of those discretionary clients, to the knowledge of Discovery Partners, Discovery Group, Mr. Donoghue or Mr. Murphy, has an economic interest in more than 5% of the class. Item 7. Identification and Classification of the Subsidiary Which Acquired the Security Being Reported on By the Parent Holding Company or Control Person Not Applicable Item 8. Identification and Classification of Members of the Group Not Applicable Item 9. Notice of Dissolution of Group Not Applicable Item 10. Certification By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect. Signature After reasonable inquiry and to the best of my knowledge and belief, I certify that the information set forth in this statement is true, complete and correct. August 17, 2006 -------------------------------------- Date DISCOVERY GROUP I, LLC, for itself and as general partner of DISCOVERY EQUITY PARTNERS, L.P. /s/ Michael R. Murphy -------------------------------------- Signature Michael R. Murphy, Managing Member -------------------------------------- Name/Title /s/ Daniel J. Donoghue -------------------------------------- Signature Daniel J. Donoghue -------------------------------------- Name/Title /s/ Michael R. Murphy -------------------------------------- Signature Michael R. Murphy -------------------------------------- Name/Title Exhibit Index Exhibit 1 Joint Filing Agreement dated as of August 17, 2006, by and between Discovery Equity Partners, L.P.; Discovery Group I, LLC; Daniel J. Donoghue; and Michael R. Murphy. EXHIBIT 1 JOINT FILING AGREEMENT The undersigned hereby agree to the joint filing of the Amendment No. 1 to the Schedule 13G to which this Agreement is attached. Dated: August 17, 2006 DISCOVERY GROUP I, LLC, for itself and as general partner of DISCOVERY EQUITY PARTNERS, L.P. By /s/ Michael R. Murphy ---------------------------------- Michael R. Murphy Managing Member /s/ Daniel J. Donoghue -------------------------------------- Daniel J. Donoghue /s/ Michael R. Murphy -------------------------------------- Michael R. Murphy